Volume 47, Number 3, 261-263, DOI: 10.1007/BF01958154

Effects of the pyrimidine-containing cytochrome P-450 inhibitor, fenarimol, on the formation of 20-OH ecdysone in flies

P. Kulcsár, B. Darvas, H. Brandtner, J. Koolman and H. H. Rees

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Abstract

The effect of fenarimol, a pyrimidine-containing cytochrome P-450 inhibitor, was tested in vitro on brain-ring gland complexes ofCalliphora vicina (Dipt., Calliphoridae), and on microsomes prepared from the fat body of 0-h wandering stage larvae ofNeobellieria bullata (Dipt., Sacrophagidae). Fenarimol had no influence on the formation of ecdysone, but it was an effective inhibitor of cytochrome P-450-dependent ecdysone 20-monooxygenase.

Key words   Calliphora vicina  -  Neobellieria bullata  - fenarimol - synthesis of 20-hydroxy ecdysone - cytochrome P-450 inhibitor - microsomal ecdysone 20-monooxygenase

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