Volume 47, Number 3, 433-436, DOI: 10.1023/B:BIOP.0000023889.20631.92

Multiple Shoot Induction and Plant Regeneration from Embryo Axes of Six Cultivars of Gossypium hirsutum

A.K. Banerjee, D.C. Agrawal, S.M. Nalawade, S. Hazra and K.V. Krishnamurthy

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Abstract

The report describes in vitro plant regeneration from embryo axis explants of six cultivars of cotton. Induction of a maximum number of multiple shoots in all six cultivars could be achieved on Murashige and Skoog's (MS) salts and Gamborg's (B5) vitamins supplemented with 0.4 μM benzyladenine (BA) and 0.1 μM napthaleneacetic acid (NAA). Elongated shoots could be rooted on half strength medium supplemented with 0.5 μM NAA. Rooted shoots survived (92 %) after hardening in the greenhouse and grew to maturity (100 %) after transfer to field.

benzyladenine - cotton - micropropagation - napthaleneacetic acid

This revised version was published online in July 2006 with corrections to the Cover Date.

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