Native rhizobia associated with
Trifolium albopurpureum, T. bifidum, T. ciliolatum, T. depauperatum, T. dichotomum, T. flavulum, T. melanthum, T. microcephalum,
T. microdon, T. oliganthum and
T. tridentatum were found in Northern California range soils. These rhizobia nodulate subterranean clover but are ineffective in nitrogen
fixation with this host. Native rhizobia compet with those in commercial inoculants to form nodules. To ensure effective nodulation
by nitrogen fixing rhizobia, commercial inoculants should be applied at rates greater than those recommended by the manufacturerse
Effective nodulation was achieved by an application of 7.5×10
4 rhizobia per seed, four times the recommended rate.