A simple and inexpensive particle acceleration apparatus was designed for direct delivery of DNA to plant cells. The Particle
Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles directly in a helium steam. High levels of transient
expression of the
β-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of maize and soybean, and leaf
tissue of cowpea. Stable transformation of soybean and maize has also been obtained using this bombardment apparatus.
Key words embryogenic suspension culture -
Glycine max
- particle bombardment - stable transformation - transient expression -
Zea mays