Imaging beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic beta cells

Subhadra C. Gunawardana, Manami Hara, Graeme I. Bell, W. Steven Head, Mark A. Magnuson and David W. Piston

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Abstract

We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.

Key words  in vitro culture - pancreatic explants - mouse - GFP-labeled - real-time

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