An actively controlled wind tunnel equipped with multiple fansand airfoils has been developed, mainly for the purpose of reproducing the atmospheric boundary layer (ABL) for wind engineering applications. Various fluctuating flows can be achieved in this wind tunnel by altering the input data of the fans and airfoils through computer control. In this study, the ABL is physically simulated in this wind tunnel, and particular attention ispaid to the simulation of the profile of Reynolds stress. The method of generating the fluctuating flow and the experimental results of reproducing the ABL are presented. As the results show, the spatial distribution of Reynolds stress is satisfactorily simulated, and the profiles of other statisticalturbulent parameters, such as mean velocity, turbulent intensity, integral scale and power spectrum are successfully reproduced simultaneously.