Benzisoxazole-3-acetic acid, a new synthetic growth regulator, was administered to protoplast cultures from
Nicotiana tabacum and subsequently to the developed microcalluses, to test its activity on plant regeneration from protoplasts in different culture conditions. Such activity, compared to that of naphthalene-acetic acid, proved to be rather low in the stage of cellular division and microcallus formation but particulary high in the stage of shoot induction from microcallus, thus confirming that the activity of this compound is mainly morphogenetic.
Keywords Auxin - benzisoxazole-3-acetic acid - protoplasts - plant regeneration
Communicated by M. R. Davey