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Second International Workshop on Bioinformatics Research and Applications (IWBRA06)

Pooling Evidence to Identify Cell Cycle–Regulated Genes

Gaolin ZhengContact Information, Tom MilledgeContact Information, E. Olusegun GeorgeContact Information and Giri NarasimhanContact Information

(1)  Bioinformatics Research Group (BioRG), School of Computer Science, Florida International University, Miami, Florida, 33199, USA
(2)  Department of Mathematical Sciences, University of Memphis, Memphis, TN 38152, USA
Abstract
Most of the biological studies have embraced statistical approaches to make inferences. It is common to have several independent experiments to test the same null hypothesis. The goal of research on pooling evidence is to combine the results of these tests to ask if there is evidence from the collection of studies to reject the null hypothesis. In this study, we evaluated four different pooling techniques (Fisher, Logit, Stouffer and Liptak) to combine the evidence from independent microarray experiments in order to identify cell cycle-regulated genes. We were able to identify a better set of cell cycle-regulated genes using the pooling techniques based on our benchmark study on budding yeast (Saccharomyces cerevisiae). Our gene ontology study on time series data of both the budding yeast and the fission yeast (Schizosaccharomyces pombe) showed that the GO terms that are related to cell cycle are significantly enriched in the cell cycle-regulated genes identified using pooling techniques.

Contact Information Gaolin Zheng
Email: gzhen001@cis.fiu.edu

Contact Information Tom Milledge
Email: tmille01@cis.fiu.edu

Contact Information E. Olusegun George
Email: eogeorge@memphis.edu

Contact Information Giri Narasimhan
Email: giri@cis.fiu.edu
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