It is often suggested that patella tracking after total knee arthroplasty (TKA) with an asymmetrical patella groove is more physiological than with a symmetrical patella groove. Therefore, this study tried to address two questions: what is the effect of TKA on patella tracking, and is patella tracking after asymmetrical TKA more physiological than patella tracking after symmetrical TKA? The patellar and tibial kinematics of five cadaveric knee specimens were measured in the intact situation, after the incision and suturing of a zipper, and after placement of a symmetrical TKA and an asymmetrical TKA, respectively. The patellae were not resurfaced. The flexion-extension kinematics were measured with an internal and external tibial moment to determine the envelope of motion (laxity bandwidth) of the tibio-femoral and patello-femoral articulation. The kinematics after TKA showed statistically significant changes in comparison to the intact situation: patellar medio-lateral translation, patellar tilt and tibial rotation were significantly affected. No statistically significant differences in knee kinematics were found between the symmetrical and the asymmetrical TKAs. We conclude that conventional TKA significantly changes physiological patello-femoral kinematics, and TKA with an asymmetrical patella groove does not improve the non-physiological tracking of the patella.