With the rapid development of the internet, e-Learning is playing an important role to life-long learning. But the phenomenon that e-Learning is lack of Knowledge Building at large has raised the attention of educational circles. This paper presents an idea that Knowledge Building Classroom based on Web2.0 as a solution to e-Learning. Based on this idea, we design and implement the e-Learning which introduces Knowledge Building Community theory and web 2.0 technologies. By the end of this paper, we take “Laboratory Inquiry” as an example to demonstrate the advantages of Knowledge Building Classroom based on Web2.0.