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Abstract

Derivatives of potato (Solanum tuberosum cv.'s lsquoMaris Bardrsquo and lsquoDesireersquo) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four lsquoMaris Bardrsquo plants transformed with pRAL1834 T-DNA and two lsquoDesireersquo plants with pBIN6 T-DNA. The transformed lsquoMaris Bardrsquo plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed lsquoDesireersquo plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin.

Key words  Genetic manipulation - Neomycin phosphotransferase - Mixed infection - Somaclonal variation -  Solanum tuberosum

Communicated by R. Riley

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