Volume 217, Number 1, 182-184, DOI: 10.1007/BF00330960

Cloning and nucleotide sequence of the Salmonella typhimurium LT2 gnd gene and its homology with the corresponding sequence of Escherichia coli K12

Peter Reeves and Gordon Stevenson

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Abstract

The complete nucleotide sequence of the Salmonella strain LT2 gnd gene for 6-phosphogluconate dehydrogenase was determined. The gene contains 1404 bases and encodes a 468 amino acid polypeptide, which is the same as for Escherichia coli K12. The DNA sequence shows 14.8% difference between the two and the amino acid sequence 3.6% difference. Changes are mostly in the third codon base and most of the amino acid changes are conservative.

Key words   gnd  - 6-Phosphogluconate dehydrogenase - DNA sequence -  Salmonella

Communicated by J.W. Lengeler

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