The effects of NAA/BA concentrations and GA₃ supply, on the development of meristem-tips excised from microshoots of one Dioscorea cayenensis-D. rotundata cultivar and of one D. praehensilis genotype, have been examined. Three NAA/BA concentrations and one addition of GA₃ to one medium were compared after 3, 8 and 11 months of culture without subculturing. Culture support was a superimposed agar/agarose (5 v/1v); growth regulators were used in a MS medium with agarose as top layer support. For the two clones, rooting was significantly decreased by the highest NAA/BA concentrations, and budding was independent of treatments. Shoot elongation was more associated with lower NAA/BA concentrations (Dioscorea cayenensis-D. rotundata) or independent (D. praehensilis). Callusing appeared more linked to higher NAA/BA concentrations (D. praehensilis) or independent Dioscorea cayenensis-D. rotundata). GA₃ supply did not improve regeneration. The genotypic differences are marked by a better survival and a better regeneration into rooted plantlets for the cultivar.