The pupil localization algorithm is very important for a face recognition system. Traditional pupil localization algorithms are easy to be affected by uneven illuminations and accessories. Aiming at those limitations, a novel pupil localization algorithm is proposed in this paper. The algorithm firstly implements face image tilt adjustment and extracts eye region through the horizontal intensity gradient integral projection and Gabor filtering. Then in order to increase the eye detection accuracy, PCA is applied to select Gabor filter, and the projection enhancement algorithm is presented. At last the Negative Radial Symmetry is presented to locate the pupil position precisely in eye windows. Experimental results show that the method can locate the pupil position accurately, and demonstrate robustness to uneven lighting, noise, accessories and pose variations.