A technique is suggested for preparing paraffin sections from tissues fixed with acetone which can be used for the immunohistochemical detection of antigens which differ in their chemical nature, including
-fetoprotein, antigens of mouse leukemia viruses, alcohol-soluble antigens of hepatocyte membranes, and certain phospholipids.
Key Words
Immunofluorescence
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localization of antigens
Laboratory of Immunochemistry and Diagnosis of Tumors, N. F. Gamaleya Institute of Epidemiology and Microbiology, Academy of Medical Sciences of the USSR. Laboratory of Pathological Anatomy, N. N. Burdenko Institute of Neurosurgery, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR G. V. Vygodchikov.) Translated from Byulleten'' Éksperimental''noi Biologii i Meditsiny, Vol. 82, No. 8, pp. 1018–1020, August, 1976.