Purpose. To examine the disposition of fexofenadine in the isolated perfused rat liver and the influence of erythromycin and dibromosulphthalein (DBSP) on the hepatic uptake and biliary excretion of fexofenadine.
Methods. Livers from four groups of rats were perfused in a recirculatory manner with fexofenadine HCl added as a bolus (125, 250, 500, or 1000
g) to perfusate. Livers from another three groups of rats were perfused with 250
g of fexofenadine HCl. With one group as control, erythromycin (4.0
g/ml) or DBSP (136
g/ml) was added to the perfusate of the other groups. In all experiments, perfusate and bile were collected for 60 min; in addition, livers from the second experiment were retained for assay. Fexofenadine was determined in perfusate, bile, and homogenized liver by HPLC.
Results. The area under the curve (AUC) of fexofenadine was linearly related to concentration. It was unchanged from control (12,800 ± 200 ng·h/ml) by erythromycin (14,400 ± 2000 ng·h/ml), but was increased 95% by DBSP (25,000 ± 2600 ng·h/ml, P <0.001). the="" ratios="" of="" the="" concentrations="" of="" fexofenadine="" in="" liver/perfusate="" were="" decreased="" significantly="" by="" dbsp;="" those="" for="" bile/liver="" were="" increased="" by="">
Conclusions. Erythromycin reduced the canalicular transport of fexofenadine into bile, whereas DBSP reduced uptake across the sinusoidal membrane.
fexofenadine - hepatic transport - inhibition - erythromycin - dibromosulphothalein