Abstract.  We examined landscape predictors of dissolved organic matter (DOM) concentration, molecular weight (M_w), and molar absorptivity at 280 nm (ɛ₂₈₀) in 60 streams from the Ontonagon River watershed in northern Michigan. During our sampling period (September 19–22, 2002), DOM concentration ranged from 4 to 35 mg C L⁻¹ across streams. DOM M_w and ɛ₂₈₀ also showed considerable variation among streams. Multiple factor regression showed that stream DOM concentrations were related to watershed area, mean watershed slope, and the percentage of watershed area in certain types of land cover (lake, total wetlands, emergent wetlands, and lowland conifer forests). Streams with higher DOM concentration also had higher DOM M_w and molar absorptivity. Moreover, DOM M_w and ɛ₂₈₀ were negatively related to the % lake and positively related to the % total wetlands in the watershed. In general, landscape variables explained more among stream variation in DOM concentration than in DOM M_w or ɛ₂₈₀ in this watershed. It thus appears that the many biogeochemical processes controlling DOM input, transportation, and degradation weaken relationships between stream DOM composition and terrestrial organic matter dynamics in this relatively large watershed. Our results indicate that the total proportion of wetlands alone may be inadequate to predict DOM concentration or physicochemistry in streams flowing from large watersheds of variable morphology and land cover composition.